Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Validation of Knockdown Efficiency of ATP10A and DBI (A and B) Western blot analysis confirming the knockdown efficiency of ATP10A and DBI in the SW620 and DLD-1 cell lines. (C–F) qRT-PCR validation of ATP10A and DBI knockdown efficiency in the SW620 and DLD-1 cell lines. Data are presented as the mean ± SD. Comparisons among four groups were analyzed by one-way ANOVA followed by Tukey post hoc test (C–F). Significance levels were denoted as follows: p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗).

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Biomarker Discovery, Knockdown, Western Blot, Quantitative RT-PCR

Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Effects of ATP10A and DBI Knockdown on CRC Cell Proliferation (A and B) CCK-8 assay investigating the influence of ATP10A and DBI knockdown on SW620 cell proliferation. (C and D) CCK-8 assay assessing the influence of ATP10A and DBI knockdown on DLD-1 cell proliferation. (E and F) EdU assay exploring the influence of ATP10A and DBI knockdown on SW620 cell proliferation; scale bar, 100 μm. (G and H) EdU assay evaluating the effects of ATP10A and DBI knockdown on DLD-1 cell proliferation; scale bar, 100 μm. Data are presented as the mean ± SD. Comparisons were analyzed using either two-way (A–D) or one-way (E–H) ANOVA, as appropriate, followed by Tukey post hoc test. Significance levels were denoted as follows: p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗); “ns” indicates no statistically significant difference.

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Knockdown, CCK-8 Assay, EdU Assay

Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Effects of ATP10A and DBI Knockdown on Genes Required for Neutrophil Recruitment and N2 Polarization (A–D) Changes in the expression of IL-8 and TGF-β1 following ATP10A knockdown in SW620 and DLD-1 cell lines. (E–H) Changes in the expression of IL-8 and TGF-β1 following DBI knockdown in SW620 and DLD-1 cell lines. Data are presented as the mean ± SD. Comparisons among four groups were analyzed by one-way ANOVA followed by Tukey post hoc test. Significance levels were denoted as follows: p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗).

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Knockdown, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Absorption and Metabolism of Urolithin A and Ellagic Acid in Mice and Their Cytotoxicity in Human Colorectal Cancer Cells

doi: 10.1155/2023/8264716

Figure Lengend Snippet: Cytotoxicity of urolithin A in HT-29, HCT 116, SW480, and SW620 cells. (a) Each cell viability was compared with its own control. ∗ p < 0.05 and ∗∗∗ p < 0.001. (b) The photographs show cells that were treated with or without 75 µ M urolithin A for 24 h. The yellow scale bar is 100 µ m.

Article Snippet: There were 4 cell lines used in this study including SW480, SW620, HCT 116, and HT-29 cells (Bioresource Collection and Research Center, Hsinchu, Taiwan).

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Absorption and Metabolism of Urolithin A and Ellagic Acid in Mice and Their Cytotoxicity in Human Colorectal Cancer Cells

doi: 10.1155/2023/8264716

Figure Lengend Snippet: Cytotoxicity of urolithin B in HT-29, HCT 116, SW480, and SW620 cells. (a) Each cell viability was compared with its own control. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. (b) The photographs show cells that were treated with or without 75 µ M urolithin B for 24 h. The yellow scale bar is 100 µ m.

Article Snippet: There were 4 cell lines used in this study including SW480, SW620, HCT 116, and HT-29 cells (Bioresource Collection and Research Center, Hsinchu, Taiwan).

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Absorption and Metabolism of Urolithin A and Ellagic Acid in Mice and Their Cytotoxicity in Human Colorectal Cancer Cells

doi: 10.1155/2023/8264716

Figure Lengend Snippet: Effect of ellagic acid on cell viability in HT-29, HCT 116, SW480, and SW620 cells. Each cell viability was compared with its own control. ∗∗∗ p < 0.001.

Article Snippet: There were 4 cell lines used in this study including SW480, SW620, HCT 116, and HT-29 cells (Bioresource Collection and Research Center, Hsinchu, Taiwan).

Techniques: Control